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nature : also known as Rong cell. Broken cell or a cell of methods, including chemical and enzymatic solution MOC. Lytic enzyme (dissolved cell) is the use of enzyme decomposition damage on the wall of the special keys, thus achieving broken, for example, the most widely used lysozyme, it concentrated on the decomposition of the cell wall glycoproteins of β - 1, 4 glycosidic bond, lipopolysaccharide dissociation, lysozyme treatment, the cells moved hypotonic solution so cell rupture. When the enzyme solution needs to select appropriate enzymes and enzyme systems, and to determine the specific reaction conditions including surcharges other ways; Chemical bacteriolysis [dissolved Cellular] (P <0.05 chemical) is the use of acid, alkali and other chemicals surfactant protein hydrolysis reagents, cell lysis or some components of leakage from the cells come back, as derived from the cell components. As for the differences intracellular amylase, adding 0.1% sodium dodecyl sulfate, or 0.4% sodium dodecyl sulfate, or 0.4% TrionX-100 enzyme solution to make surfactant, 30 ° Coscillation 30h, allogeneic amylase can extract more out completely. However, the chemical solution MOC existence surfactant pollutants, acid, alkali and other selective non-sensitive as well as protein and enzyme destructive role weaknesses.
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