The present invention relates to a process of acid hydrolyzing ginsenoside to prepare ginsenoside Rg3. By means of utilizing the characteristic that in acid environment, the glucosyl in ginsenoside 20-hydroxyl group has lower stability than that in 3-hydroxyl group and through adding protecting agent and inert gas, controlling reaction temperature and acid concentration and other technological measures, the present invention solves the problems of directionally hydrolyzing ginsenoside in acid hydrolyzing condition, and prepares ginsenoside Rg3. The present invention makes it possible to prepare ginsnoside Rg3 in simple process, low cost and great amount so as to meet the requirement of providing medicinal resource for anticancer medicines. |