| Original document(30 pages) 中文版 |
The present invention relates to a method of separating, purifying and recombining thioredoxin fusion protein from colibacillus. The method includes suspending colibacillus containing thioredoxin fusion protein in breaking liquid with proper pH value, breaking colibacillus and centrifugalizing to obtain precipitant; dissolving the precipitant in urea solution in proper concentration and centrifugalizing to obtain supernatant containing thioredoxin fusion protein. The present invention also provides similar method of preparing recombinant exogenous protein. The present invention is suitable for large-scale production of recombinant exogenous protein. |
Application Number
申请号 |
01132165 |
Application Date
申请日 |
2001.11.09 |
| Title 名称 |
Method of separating, purifying and recombining thioredoxin fusion protein from colibacillus and uts application
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Publication Number
公开号 |
1417226 |
Publication Date
公开日 |
2003.05.14 |
| Approval Pub. Date |
|
Granted Pub. Date |
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| International Classification 分类号 |
C07K1/14,C07K14/265 |
Applicant(s) Name
申请人 |
5Shanghai Hanyin Pharmaceutical Industry Co., Ltd. |
| Address 地址 |
201324 |
| Inventor(s) Name 发明人 |
Zhao Bin, Xu Lin |
| Attorney & Agent 代理人 |
huang renmin |
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