Original document(5 pages) Authorized document(5 pages) 中文版
    The preparation process of strong fluorescent phycocyanin using fresh smashed algae as raw material includes: washing with EDTA containing phosphoric acid buffering solution, ultrasonic crushing of cell to release pigment protein, centrifugal separation to eliminate insoluble cell membrane and organelle and to collect the supernatant; salting out phycocyanin precipitant; dissolving the precipitant in EDTA containing phosphoric acid buffering solution, desalting with Sephadex G-25, eliminating alkali impurity protein in DEAE-Sepharose FF column; obtaining phycocyanin eluent in hydroxyapatite column; eliminating acid impurity protein in CM-Sepharose FF column; and finally obtaining high-purity strong fluorescent phycocyanin in hydroxyapatite column. The process is suitable for large-scale industrial production.
Application Number
申请号		 02150919 Application Date
申请日		 2002.11.28
Title 名称 Process of preparing strong fluorescent phycocyanin
Publication Number
公开号		 1417227 Publication Date
公开日		 2003.05.14
Approval Pub. Date Granted Pub. Date 2004.09.15
International Classification 分类号 C07K1/14,C07K14/405
Applicant(s) Name
申请人		 Zhejiang Univ.
Address 地址 310027
Inventor(s) Name 发明人 Gong Xingguo, Zeng Dongyun
Attorney & Agent 代理人 han gemei

  
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