The invention relates to an alpha-interferon plasmid DNA-liposome preparation making method. It sub-clones the alpha-interferon plasmid DNA in true nucleus expression carrier pSG5 to make expression plasmid, then mixes dilaurin (GDL), cholesterin (CH), polyoxyethylene stearate (POE-10), polyvinyl -pyrrolidone (PVP) and alpha-tocopherol to dissolve at 70 deg.C, filters by 0.22mum microhole film, then heats to 70 deg.C, slowly drips 70 deg.C aseptic water in the lipid mixture to make the total lipid concentration not less than 1g/ml, quickly evenly mixes two phases to cool to room temperature, then stores the liposome at 4 deg.C for ultrasonic processing at room temperature, adds in equal bulk of water solution with the expression plasmid, and evenly mixes to hatch to obtain the preparation. |