The present invention relates to the crossed field of nano technology and biomedicine, and is the preparation process of composite nano quantum dot particle with adjustable fluorescent spectrum. In a water-in-oil microemulsion system, two or more kinds of nano quantum dot particles with distinguishable fluorescent colors are included simultaneously into nano silica particle to form one kind of composite nanometer quantum dot particle with granularity of 10-100 nm and adjustable fluorescent spectrum. The composite nano quantum dot particle has its fluorescent peak position and strength adjusted via altering the sort and proportion of the quantum dots of different fluorescent colors. The composite nano quantum dot particle may be used as biological fluorescent probe for various biomedicine fields. |